Extracellular Production of Yeast Protease

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Production of Extracellular Protease and Determination of Optimal Condition by Bacillus Licheniformis BBRC 100053 (RESEARCH NOTE)

The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...

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Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate Salicola marasensis

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Regulation of extracellular protease production in Bacillus cereus.

Both sporulation and protease production can be inhibited by growing Bacillus cereus T in a medium containing a high concentration of a mixture of amino acids. Mutants selected for the ability to sporulate in this inhibitory medium were found to produce high levels of protease in the normal and inhibitory media. Comparison of the mutant and wild-type enzymes by gel electrophoresis and heat inac...

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Production of a Thermostable Extracellular Protease from Thermophilic Bacillus Species

Isolation and identification of thermophilic Bacillus sp was carried out from soil samples of a tannery area. The isolate KCPSS-3, when cultivated in liquid cultures containing trisodium citrate, produced protease. The production reached a maximum in 9 h, with levels of 2.15 U/mg protein. The microorganism utilized several carbon sources for the production of protease. Starch was found to be th...

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Enhanced Production and Characterization of a Highly Stable Extracellular Protease from an Extreme Halophilic Isolate Salicola marasensis

Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of Salicola marasensis, an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was ...

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1971

ISSN: 0002-1369

DOI: 10.1080/00021369.1971.10860124